The first platform that maps real-time protein signaling to predict CAR-T performance.

QMI is a high-throughput proteomic assay that measures real-time protein-protein interactions in living cells. By quantifying interactions between 400+ signaling proteins across CAR and TCR pathways, QMI reveals how different constructs activate, persist, or fail.

How It Works

Sample Prep

Isolate primary T cells transduced with your CAR construct.

Stimulation

Expose cells to target antigen or control stimuli.

QMI Assay

Multiplexed co-immunoprecipitation + bead-based detection for 400+ protein interactions.

Analysis

Clustered interaction modules, PCA, and signature profiles compared across constructs or conditions.

A CD28 and TRAF module is increased in CRS-producing CARs

A CD28 and TRAF module is increased in CRS-producing CARs.

Protein Networks That Define Performance

  • Activation Signature
    Identify early activation cascades across multiple protein networks.

  • Safety & Exhaustion Indicators
    Detect markers of tonic signaling, overactivation, or pathway shutdown.

  • Domain Sensitivity
    Compare signaling outputs from hinge, TM, linker, or co-stimulatory domain variants.

  • Construct Discrimination
    Resolve subtle but important differences that other assays miss.

Advantages of QMI

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Network-Level Insights

QMI quantifies protein-protein interactions across signaling hubs, revealing activation and exhaustion pathways invisible to bulk cytokine or flow assays.

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Predictive Safety Signatures

Detect biosignatures associated with exhaustion, hyperactivation, or cytokine release syndrome — well before clinical dosing.

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High-Throughput Multiplexing

Our panel spans scaffold proteins, kinases, adaptors, and co-stimulatory effectors — enabling comprehensive construct profiling in a single assay run.

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Quantitative & Visual Output

We deliver PCA plots, heatmaps, and clustered signaling modules — not just raw readouts — so you can act on the data immediately.

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Modular & Customizable

Select specific nodes (e.g., CD28, TRAF2, SHP2) or add custom targets to fit your construct’s biology or therapeutic goals.

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Rapid Turnaround for Construct Screening

QMI enables fast, iterative optimization — ideal for side-by-side construct comparison and pre-IND validation workflows.